The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample.Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 35 l. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)). TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. For more information, please contact our Technical Service team at cellculturesupport@lifetech.com. The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc.The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead cDNA was synthesized with SuperScript III, and qPCR was performed with Fast SYBR Green Master Mix (Thermo Fisher). Plasmid DNA was extracted from the sampled cells using the Zymoprep Yeast Plasmid MiniPrep II kit (Zymo Research #D2004) but with a modified first step where cell pellets were spheroblasted with 10 U/ml zymolase (amsbio #1204911) in sorbitol buffer (1 M Sorbitol, 100 mM EDTA pH 8, 14 mM beta-mercaptoethanol). All bead beater tubes were fitted with a 2 mL tube holder assembly and processed at maximum speed for 30 min. The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc.The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead Fibroblast total RNA was isolated from FACS-sorted cells with TRIzol (Thermo Fisher Scientific) followed by extraction using the Direct-Zol RNA Miniprep Kit (Zymo Research). The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. The procedure is easy. Model fern reveals insight into DNA thievery in ferns Open Green Genomes hits milestone with C. richardii The Science Fern genomes are huge, and incredibly complex making it difficult to grasp a deeper understanding of fundamental aspects of fern biology and, more broadly, land plant evolution. The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa.The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our We recently shut down crispr.mit.edu, but there are many other guide design tools available that we hope you will find helpful. The procedure for these RNA purification kits is easy: simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutral Thank you to the thousands of users who visited our guide design tool over the past five years. Cellular RNA was isolated with a Zymo Direct-zol RNA Miniprep kit. To establish a robust protocol for generation of UB structures these cells also expressed KIT and CXCR4 Total RNA was isolated using Direct-zol RNA Miniprep (Zymo Research, R2051). TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa.The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our The unique spin-column design also provides zero buffer retention and a low elution volume. The solution was initially used and published by Piotr Chomczyski and Nicoletta Sacchi in 1987.. TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies, and Tri-Reagent is the brand name from MRC, which was founded by The formulations are identical, made with slightly different yeastolates derived from the same species. Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free kits from Supplier Q and Supplier MN. All bead beater tubes were fitted with a 2 mL tube holder assembly and processed at maximum speed for 30 min. The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. ZymoPUR ZymoPUR Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)). The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in No phase separation, precipitation, or post-purification steps are necessary. The solution was initially used and published by Piotr Chomczyski and Nicoletta Sacchi in 1987.. TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies, and Tri-Reagent is the brand name from MRC, which was founded by Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources ( No phase separation, precipitation, or post-purification steps are necessary. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)). 11720-067 and 11720-034. For denaturation and purification, urea (Sigma) was added to a final concentration of 2.5 M, samples were incubated for 30 min, and RNA was purified using a Direct-Zol RNA miniprep (Zymo Research). Cellular RNA was isolated with a Zymo Direct-zol RNA Miniprep kit. The procedure is easy. The formulations are identical, made with slightly different yeastolates derived from the same species. cDNA was synthesized with SuperScript III, and qPCR was performed with Fast SYBR Green Master Mix (Thermo Fisher). We recently shut down crispr.mit.edu, but there are many other guide design tools available that we hope you will find helpful. Thank you to the thousands of users who visited our guide design tool over the past five years. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. ZymoPUR All bead beater tubes were fitted with a 2 mL tube holder assembly and processed at maximum speed for 30 min. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutral Fibroblast total RNA was isolated from FACS-sorted cells with TRIzol (Thermo Fisher Scientific) followed by extraction using the Direct-Zol RNA Miniprep Kit (Zymo Research). The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample.Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 35 l. For denaturation and purification, urea (Sigma) was added to a final concentration of 2.5 M, samples were incubated for 30 min, and RNA was purified using a Direct-Zol RNA miniprep (Zymo Research). [] Direct-zol RNA MiniPrep Kit: Zymo research : Direct & Efficient Fast 10 min. Plasmid DNA was extracted from the sampled cells using the Zymoprep Yeast Plasmid MiniPrep II kit (Zymo Research #D2004) but with a modified first step where cell pellets were spheroblasted with 10 U/ml zymolase (amsbio #1204911) in sorbitol buffer (1 M Sorbitol, 100 mM EDTA pH 8, 14 mM beta-mercaptoethanol). Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free kits from Supplier Q and Supplier MN. The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in Plasmid DNA (pGL3 ) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturers suggested protocol (in duplicate). Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources ( or Quick-RNA MiniPrep from Zymo Research. Purified genomic DNA for CRISPRCas9 knockout screens was amplified using Touchdown PCR. Citations Reviews I did an assay using RNA/DNA shield and it works perfect. 11720-067 and 11720-034. The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc.The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. To establish a robust protocol for generation of UB structures these cells also expressed KIT and CXCR4 Total RNA was isolated using Direct-zol RNA Miniprep (Zymo Research, R2051). Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, For denaturation and purification, urea (Sigma) was added to a final concentration of 2.5 M, samples were incubated for 30 min, and RNA was purified using a Direct-Zol RNA miniprep (Zymo Research). Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources ( The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. The procedure is easy. Citations Reviews I did an assay using RNA/DNA shield and it works perfect. Genomic DNA was isolated using the Genomic DNA Isolation Kit (Zymo Research; ZD3025). prep No phase separation No precipitation No phenol carryover: 1 : R2050 protocol.pdf (432 KB) 2 : R2050 datasheet.pdf (2.72 MB) 3 : Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutral or Quick-RNA MiniPrep from Zymo Research. 11720-067 and 11720-034. [] Direct-zol RNA MiniPrep Kit: Zymo research : Direct & Efficient Fast 10 min. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. [] Direct-zol RNA MiniPrep Kit: Zymo research : Direct & Efficient Fast 10 min. Informational: This product is the replacement for Catalog No. Purified genomic DNA for CRISPRCas9 knockout screens was amplified using Touchdown PCR. The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in Plasmid DNA was extracted from the sampled cells using the Zymoprep Yeast Plasmid MiniPrep II kit (Zymo Research #D2004) but with a modified first step where cell pellets were spheroblasted with 10 U/ml zymolase (amsbio #1204911) in sorbitol buffer (1 M Sorbitol, 100 mM EDTA pH 8, 14 mM beta-mercaptoethanol). The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Informational: This product is the replacement for Catalog No. Plasmid DNA (pGL3 ) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturers suggested protocol (in duplicate). With these RNA purification kits, no phase separation, precipitation, or post-purification steps are necessary. Purified genomic DNA for CRISPRCas9 knockout screens was amplified using Touchdown PCR. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. No phase separation, precipitation, or post-purification steps are necessary. The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample.Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 35 l. Thank you to the thousands of users who visited our guide design tool over the past five years. Plasmid DNA (pGL3 ) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturers suggested protocol (in duplicate). The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Model fern reveals insight into DNA thievery in ferns Open Green Genomes hits milestone with C. richardii The Science Fern genomes are huge, and incredibly complex making it difficult to grasp a deeper understanding of fundamental aspects of fern biology and, more broadly, land plant evolution. Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free kits from Supplier Q and Supplier MN. The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. To establish a robust protocol for generation of UB structures these cells also expressed KIT and CXCR4 Total RNA was isolated using Direct-zol RNA Miniprep (Zymo Research, R2051). Informational: This product is the replacement for Catalog No. cDNA was synthesized with SuperScript III, and qPCR was performed with Fast SYBR Green Master Mix (Thermo Fisher). The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. With these RNA purification kits, no phase separation, precipitation, or post-purification steps are necessary. The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Citations Reviews I did an assay using RNA/DNA shield and it works perfect. Model fern reveals insight into DNA thievery in ferns Open Green Genomes hits milestone with C. richardii The Science Fern genomes are huge, and incredibly complex making it difficult to grasp a deeper understanding of fundamental aspects of fern biology and, more broadly, land plant evolution. The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 g (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. For more information, please contact our Technical Service team at cellculturesupport@lifetech.com. With these RNA purification kits, no phase separation, precipitation, or post-purification steps are necessary. The cell lines were authenticated by short-tandem-repeat DNA profiling. TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. Genomic DNA was isolated using the Genomic DNA Isolation Kit (Zymo Research; ZD3025). The cell lines were authenticated by short-tandem-repeat DNA profiling. The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa.The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our The unique spin-column design also provides zero buffer retention and a low elution volume. The procedure for these RNA purification kits is easy: simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. or Quick-RNA MiniPrep from Zymo Research. We recently shut down crispr.mit.edu, but there are many other guide design tools available that we hope you will find helpful. The procedure for these RNA purification kits is easy: simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. For more information, please contact our Technical Service team at cellculturesupport@lifetech.com. prep No phase separation No precipitation No phenol carryover: 1 : R2050 protocol.pdf (432 KB) 2 : R2050 datasheet.pdf (2.72 MB) 3 : The cell lines were authenticated by short-tandem-repeat DNA profiling. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, prep No phase separation No precipitation No phenol carryover: 1 : R2050 protocol.pdf (432 KB) 2 : R2050 datasheet.pdf (2.72 MB) 3 : The solution was initially used and published by Piotr Chomczyski and Nicoletta Sacchi in 1987.. 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